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DNA amplifications were performed in a Bio-Rad CFX-96 real-time thermocycler (Bio-Rad, USA), running a standard program (95C for 2 min, 40 cycles at 95C for 10 s, 60C for 10 s and 72C for 30 s, followed by a melting curve analysis to determine amplicon specificity using a temperature range from 65 to 95C with increments of 0.5C)
In-house volunteers who donated blood for test development and validation were from our local community and recruited through an advertisement
Competitive athletes should not use it
Once treatment is discontinued, the skin may gradually return to its natural state over time
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