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In the last 2 h of the 24-h permeability assays, the acceptor compartments were supplemented with Hoechst 33342 dye (2 g/ml) to label the cell nuclei of organoids

Final concentration: 15 mg 1.5 mL = 10 mg/mL Practical conversion: 0.1 mL = 1 mg 0.25 mL = 2.5 mg 0.5 mL = 5 mg 0.75 mL = 7.5 mg 1.0 mL = 10 mg This dilution makes weekly titration easier