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Moreover, the T 50 for each protein was calculated as the logarithm (base 2) over the corrected T 50 ratio between 5xFAD and WT mice

Generation and quantification of persulfides on Cofilin1 Purified full-length human recombinant Cofilin 1 protein expressed in Escherichia coli (Abcam #62958) (11 g/sample) was incubated with GSH (1 mM), MitoPerSulf (100 M) or both in 50 mM HEPES buffer (pH 7.4) for 7 min at RT, followed by alkylation with IAM-TPP (20 mM) at RT for 30 min in the dark

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As the population of diabetic patients is continuously expanding around the world, the demand for effective glucose-lowering treatments is magnifying significantly